Technical Reference #693

693.

Modulation Of Glioma Cell Migration And Invasion Using Cl And K+ Ion Channel Blockers Liliana Soroceanu; Timothy J. Manning Jr; and Harald Sontheimer, The University of Alabama at Birmingham, The Journal of Neuroscience, 19(693), (1999)
Link To Paper

Abstract:
Human malignant gliomas are highly invasive tumors. Mechanismsthat allow glioma cells to disseminate migrating throughthe narrow extracellular brain spaces are poorly understood.We recently demonstrated expression of large voltagedependentchloride (C

Keywords:
chlorotoxin; glioma invasiveness; chloride channel; RVD; MEQ; cell migration

Materials & Methods:
Glioma spheroids (200–300 mm) were transferred under a low magnification microscope using a sterile Pasteur pipette to 96 multiwell plates (Nunc Roskilde Denmark) and 35 mm glass bottom culture dishes (from the MatTek Corporation Ashland MA).

Microscopic Technique
Fluorescence Microscopy, Time Lapse

Cell Type(s)
U251MG, D54MGLacZ