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Technical Reference #686

Glass Bottom Culture Dishes

This study used MatTek product(s):

P35G-1.5-20-C

Citation in paper containing MatTek reference:
MatTek Corp. glass-bottom dishes (http://www.glass-bottom-dishes.com/)

686.

In Vitro Analysis Of Transneuronal Spread Of An Alphaherpesvirus Infection In Peripheral Nervous System Neurons B. Feierbach; M. Bisher; J. Goodhouse; and L. W. Enquist, Princeton University, The Journal of Virology, 81(686), (2007)
Link To Paper

Abstract:
The neurotropic alphaherpesviruses invade and spread in the nervous system in a directional manner between synaptically connected neurons. Until now this property has been studied only in living animals and has not been accessible to in vitro analysis. I

Materials & Methods:
Cells and virus strains. The swine kidney epithelial cell line PK15 was purchased from the American Type Culture Collection (CCL-22). PK15 cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum and 1% penicillin

Microscopic Technique
Transmission Electron Microscopy

Cell Type(s)
PK15