Technical Reference #674

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
MatTek dishes pretreated with poly-l-lysine

674.

Exocytotic Release Of Atp From Cultured Astrocytes Tina Pangrsic; Maja Potokar; Matjaz Stenovec; Marko Kreft; Elsa Fabbretti; Andrea Nistri; Evgeny Pryazhnikov; Leonard Khiroug; Rashid Giniatullin; and Robert Zorec, University of Ljubljana, The Journal of Biological Chemistry, 282(674), (2007)
Link To Paper

Abstract:
Astrocytes appear to communicate with each other as well as with neurons via ATP. However the mechanisms of ATP release arecontroversial. To explore whether stimuli thatincrease [Ca2+]i also trigger vesicular ATPrelease from astrocytes we labeled AT

Materials & Methods:
The cells were plated at a density of 1.5·105 cells/cm2 on MatTek dishes pre-treated with poly-L-lysine.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
E18