Technical Reference #669

669.

Characterization Of Interactions Of Adapter Protein Rapl/Nore1B With Rap Gtpases And Their Role In T Cell Migration Mandy Miertzschke; Paula Stanley; Tom D. Bunney; Fernando Rodrigues-Lima; Nancy Hogg; and Matilda Katan, The Insitute of Cancer Research, The Journal of Biological Chemistry, 282(669), (2007)
Link To Paper

Abstract:
Using a model of integrin-triggered random migration of T cells we show that stimulation of LFA-1 integrins leads to theactivation of Rap1 and Rap2 small GTPases. We further showthat Rap1 and Rap2 have distinct roles in adhesion and randommigration of

Keywords:
T Cell Migration; Adapter Protein RAPL/Nore1B; RAP GTPases

Materials & Methods:
Briefly 35-mm glass bottom microwell dishes (MatTek Corp. Ashland MA) were coated at 4 °C overnight with 3 g/ml ICAM-1Fc in phosphatebuffered saline and then blocked with 2.5% bovine serum albumin in phosphate-buffered saline

Microscopic Technique
Video Microscopy

Cell Type(s)
ICAM-1Fc