Technical Reference #660

660.

Lck-Independent Triggering Of T-Cell Antigen Receptor Signal Transduction By Staphylococcal Enterotoxins Sho Yamasaki; Makoto Tachibana; Nobukata Shinohara; and Makio Iwashima‡§i, Japan Science and Technology Corporation, The Journal of Biological Chemistry, 272(660), (1997)
Link To Paper

Abstract:
Superantigens (Sags) activate T-cells in a manner specific to the Vb region of the T-cell antigen receptor.Stimulations by SAgs provoke drastic T-cell activationthat leads to programmed cell death or the anergic stateof responding cells. To characteriz

Materials & Methods:
Fluorescent Microscopic Analysis of Intracellular Free Calcium— Cells (5 3 105) were loaded with 5 mM Fura-2/AM (Dojindo Kumamoto Japan) in Hanks’ balanced saline solution for 30 min at 37 °C and plated on a glass-bottom microwell dish (Mattek Ashland

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Jurkat