Technical Reference #658

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottom culture dish (MatTek)

658.

B Cells Productively Engage Soluble Antigen-Pulsed Dendritic Cells: Visualization Of Live-Cell Dynamics Of B Cell-Dendritic Cell Interactions Ning-Na Huang, Sang-Bae Han, Il-Young Hwang, and John H. Kehrl, National Institute of Allergy and Infectious Diseases, The Journal of Immunology, 175(658), 7126, (2005)
Link To Paper

Abstract:
Interactions between B lymphocytes and Ag-bearing dendritic cells (DC) likely occur at inflammatory sites and within lymphoid organs. To better understand these interactions we imaged B cells (TgB) from hen egg lysozyme (HEL) transgenic mice and DC pulsed

Materials & Methods:
Three-dimensional collagen matrix culture and cell collection Purified B cells and DC were labeled with Vybrant green CFDA SE (carboxy-fluorescein diacetate, succinimidyl ester) or Vybrant red DiI (Molecular Probes), respectively, following the manufactu

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Purified B cells and DC