Technical Reference #657

657.

Fluctuation Of Chromatin Unfolding Associated With Variation In The Level Of Gene Expression Noriko Sato; Masahito Nakayama and Ken-ichi Arai, Tokyo Metropolitan Institute of Medical Science, Genes to Cells, 9(657), (2004)
Link To Paper

Abstract:
We examined whether spontaneous alteration of chromatin structure if any correlates with variation in gene expression. Gene activation is associated with changes in chromatin structure at different levels.

Materials & Methods:
FISH and immuno-FISH Cells were grown on a poly D-lysine-coated glass-bottom microwell dish (MatTek MA USA). To induce GFP-ASK expression 2 µg/mL of doxycycline was added for 18–24 h. Cells were washed with PBS and CSK buffer (10 mM PIPES; pH 6.8 100

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HeLa