Technical Reference #656

656.

Synaptotagmin Vii Is Targeted To Dense-Core Vesicles And Regulates Their Ca2+-Dependent Exocytosis In Pc12 Cells Mitsunori Fukuda; Eiko Kanno; Megumi Satoh; Chika Saegusa; and Akitsugu Yamamoto, Institute of Physical and Chemical Research, The Journal of Biological Chemistry, 279(656), (2004)
Link To Paper

Abstract:
It has recently been proposed that synaptotagmin (Syt) VII functions as a plasma membrane Ca2+ sensor for dense-core vesicle exocytosis in PC12 cells based on the results of transient overexpression studies using green fluorescent protein (GFP)-tagged Syt

Materials & Methods:
Cell Culture Immunocytochemistry and Immunoelectron Microscopy—PC12 cell lines stably expressing FLAG-Syt VII-GFP (Syt VII and GFP were separated by a Gly linker) were established as described elsewhere (40 45) (see Fig. 2A). The cells were cultured on

Microscopic Technique
Immunoelectron Microscopy

Cell Type(s)
PC12