Technical Reference #654

654.

A Comparison Of Ca2+ Channel Blocking Mode Between Gabapentin And Verapamil: Implication For Protection Against Hypoxic Injury In Rat Cerebrocortical Slices Michiko Oka; Yoshinori Itoh; Miyuki Wada; Akira Yamamoto & ;Takuya Fujita, Kyoto Pharmaceutical University, British Journal of Pharmacology, 139(654), (2003)
Link To Paper

Abstract:
The mode of Ca2+ channel blocking by gabapentin [1-(aminomethyl)cyclohexane acetic acid] was compared to those of other Ca2+ channel blockers and the potential role of Ca2+ channel antagonists in providing protection against hypoxic injury was subsequent

Keywords:
Gabapentin; hypoxic injury; lactate dehydrogenase; cGMP; nitric oxide; voltage-gated Ca2+ channels; rat cerebrocortical slices; -agatoxin IVA; -conotoxin GVIA; verapamil

Materials & Methods:
Primary neuronal culture Primary neuronal cultures were prepared from the cerebral cortex of fetal mice as described previously (Oka et al. 1999; 436 M. Oka et al Neuroprotection by gabapentin via Ca2+ channel British Journal of Pharmacology vol 139 (2)

Microscopic Technique
Inverted Microscopy

Cell Type(s)
Cerebellar