Technical Reference #652

652.

In Situ Visualization Of Messenger Rna For Basic Fibroblast Growth Factor In Living Cells Toshihiko Matsuo, Okayama University Medical School, Biochimica et Biophysica Acta, 1379(652), (1998)
Link To Paper

Abstract:
We examined whether messenger RNA for basic fibroblast growth factor bFGF.could be visualized specifically by afluorescent probe in living cells. A 15-nucleotide-long antisense or sense sequence for human bFGF was sandwichedbetween two complementary 5-n

Keywords:
Molecular beacon; Messenger RNA; Basic fibroblast growth factor; Fluorophore; Antisense probe; Trabecular cell

Materials & Methods:
2 mg of the fluorescent probe dissolved in 2 ml of water were mixed with 9ml of liposomes consisting of 0.4 mg of NNNXNX-tetramethyl-N-NX-bis 2-hy- droxyethyl.-23-dioleoyloxy-14-butanediammonium iodide and 0.3 mg of L-dioleoyl phosphatidylethanolamin

Microscopic Technique
Inverted Microscopy

Cell Type(s)
trabecular