Technical Reference #644

644.

A Specific Activation Of The Mitogen-Activated Protein Kinase Kinase 1 (Mek1) Is Required For Golgi Fragmentation During Mitosis Antonino Colanzi; Thomas J. Deerinck; Mark H. Ellisman; and Vivek Malhotra*, University of California San Diego (UCSD), The Journal of Cell Biology, 149(644), (2000)
Link To Paper

Abstract:
Abstract. Incubation of permeabilized cells with mitoticextracts results in extensive fragmentation of the pericentriolarly organized stacks of cisternae. The fragmentedGolgi membranes are subsequently dispersedfrom the pericentriolar region. We have s

Keywords:
mitogen-activated protein kinase kinase 1 • extracellular signal–regulated kinases • mitosis • Golgi fragmentation • phosphorylation

Materials & Methods:
NRK cells were grown in special petri dishes (Matteck Corporation; Takizawa et al. 1993) permeabilized as described above and processed for immunoelectron microscopy as described by Polishchuk et al.

Microscopic Technique
Immunoelectron Microscopy

Cell Type(s)
NRk