Technical Reference #641

641.

Rac1 Null Mouse Embryonic Fibroblasts Are Motile And Respond To Pdgf Luis Vidali; Feng Chen; Gregor Cicchetti; Yasutaka Ohta; and David J. Kwiatkowski, Brigham & Women's Hospital, Molecular Biology of the Cell, 17(641), (2006)
Link To Paper

Abstract:
Previous studies of Rac1 in fibroblasts have used dominant negative constructs whichmay have non-specific effects. We used a conditional Rac1 allele to critically examine Rac1function in mouse fibroblasts.

Materials & Methods:
Mouse alleles PCR and Southern blot Rac1 conditional and null alleles were generated as previously reported (Glogauer et al. 2003; Gu et al. 2003). The conditional Rac1 allele contains loxP sites flanking exon1; upon cremediated excision this allele ge

Microscopic Technique
Time Lapse Microscopy

Cell Type(s)
MEF