Technical Reference #637

637.

Migrating Fibroblasts Perform Polarized; Microtubuledependent Exocytosis Towards The Leading Edge Jan Schmoranzer; Geri Kreitzer and Sanford M. Simon, Rockefeller University, The Journal of Cell Science, 116(637), (2003)
Link To Paper

Abstract:
Cell migration might involve biased membrane traffic toward the leading edge to facilitate the building of extracellular matrix membrane protrusions and adhesion plaques. We tested the hypothesis that secretory vesicles are preferentially delivered towar

Keywords:
Total internal reflection; Evanescent wave; Cell polarization; Post-Golgi vesicle; Migration; Secretion

Materials & Methods:
Cells were plated either onto glass bottom dishes (MatTek Ashland MA) or on autoclaved coverslips (Fisher Scientific).

Microscopic Technique
Fluorescence Microscopy, Total Internal Reflection Fluorescence Microscopy

Cell Type(s)
NRk