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Technical Reference #636

Glass Bottom Culture Dishes

This study used MatTek product(s):

P35G-0-14-C

Citation in paper containing MatTek reference:
MatTek glass bottom dishes (MatTek, Ashland, MA)

636.

Histone Acetylation Increases Chromatin Accessibility Sabine M. Görisch, Malte Wachsmuth, Katalin Fejes Tóth, Peter Lichter and Karsten Rippe, Deutsches Krebsforschungszentrum, The Journal of Cell Science, 118(24), 5826, (2005)
Link To Paper

Abstract:
In eukaryotes, the interaction of DNA with proteins and supramolecular complexes involved in gene expression is controlled by the dynamic organization of chromatin inasmuch as it defines the DNA accessibility.

Keywords:
Heterochromatin, Image correlation spectroscopy, Trichostatin A, Microinjection

Materials & Methods:
HeLa cells (ATCC: CCL-2) were cultured on glass coverslips for fixed cell analysis and on MatTek glass bottom dishes (MatTek, Ashland, MA) for in vivo imaging in DMEM for 1 day.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HeLa