Technical Reference #635

635.

Role Of Microtubules In Fusion Of Post-Golgi Vesicles To The Plasma Membrane Jan Schmoranzer and Sanford M. Simon, Rockefeller University, Molecular Biology of the Cell, 14(635), (2003)
Link To Paper

Abstract:
Biosynthetic cargo is transported away from the Golgi in vesicles via microtubules. In the cell periphery the vesicles are believed to engage actin and then dock to fusion sites at the plasma membrane.

Keywords:
microtubules; Golgi vesicles; actin; plasma membrane

Materials & Methods:
Cells were plated either onto glass bottom dishes (MatTek Ashland MA) or on autoclaved coverslips (Fisher Scientific Fair Lawn NJ).

Microscopic Technique
Fluorescence Microscopy, Total Internal Reflection Fluorescence Microscopy

Cell Type(s)
NRk