|
|
| TR |
TITLE |
AUTHORS |
KEYWORDS |
MATERIALS & METHODS
|
MICROSCOPIC
TECHNIQUES |
SPECIES |
MORPHOLOGY |
CELL
LINE |
| 635 |
Role
of Microtubules in Fusion of Post-Golgi Vesicles to the Plasma Membrane |
Jan
Schmoranzer and Sanford M. Simon |
microtubules,
Golgi vesicles, actin, plasma membrane |
Cells were plated either onto
glass bottom dishes (MatTek, Ashland, MA) or on autoclaved coverslips (Fisher Scientific, Fair
Lawn, NJ).
|
total
internal reflection-fluorescence microscopy (TIR-FM) |
rat |
|
NRK,
MDCK |
| |
Biosynthetic cargo is transported away
from the Golgi in vesicles via microtubules. In the cell periphery the vesicles are believed
to engage actin and then dock to fusion sites at the plasma membrane. Using dual-color total
internal reflection fluorescence microscopy, we observed that microtubules extended within 100
nm of the plasma membrane and post-Golgi vesicles remained on microtubules up to the plasma
membrane, even as fusion to the plasma membrane initiated. Disruption of microtubules eliminated
the tubular shapes of the vesicles and altered the fusion events: vesicles required multiple
fusions to deliver all of their membrane cargo to the plasma membrane. In contrast, the effects
of disrupting actin on fusion behavior were subtle. We conclude that microtubules, rather than
actin filaments, are the cytoskeletal elements on which post-Golgi vesicles are transported
until they fuse to the plasma membrane. |
|
|
|
|
