Technical Reference #634

634.

Trichothiodystrophy Fibroblasts Are Deficient In The Repair Of Ultraviolet-Induced Cyclobutane Pyrimidine Dimers And (6–4)Photoproducts Yoko Nishiwaki, Nobuhiko Kobayashi, Kyoko Imoto, Taka-aki Iwamoto, Aya Yamamoto, Sachiko Katsumi, Toshihiko Shirai, Shigeki Sugiura, Yu Nakamura, Alain Sarasin, Sachiko Miyagawa, and Toshio Mori, Nara Medical University, Journal of Investigative Dermatology, 112, 531, (2004)
Link To Paper

Abstract:
A photosensitive form of trichothiodystrophy (TTD) results from mutations in the same XPD gene as the DNArepair- deficient genetic disorder xeroderma pigmentosum group D (XP-D).

Keywords:
CPD, 6–4PP, TTD, xeroderma pigmentosum, XPB

Materials & Methods:
Briefly, cells were cultured in 35-mm glass-bottom dishes (MatTek, Ashland, MA) for 2 days.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
MSU-1