Technical Reference #633

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm glass-bottom dishes (MatTek; Ashland; MA)

633.

Lethality To Human Cancer Cells Through Massive Chromosome Loss By Inhibition Of The Mitotic Checkpoint Geert J. P. L. Kops; Daniel R. Foltz; Don W. Cleveland, UCSD, PNAS, 101(633), (2004)
Link To Paper

Abstract:
A compromised mitotic checkpoint the primary mechanism for ensuring that each new cell receives one copy of every chromosome has been implicated as a contributor to carcinogenesis.

Keywords:
carcinogenesis; chromosomally instable colorectal cancer cells; proliferation

Materials & Methods:
HeLa cells seeded on 35-mm glass-bottom dishes (MatTek Ashland MA) were transfected with pH2BEYFP and the indicated siRNA plasmids in a ratio of 1:10.

Microscopic Technique
Inverted Microscopy

Cell Type(s)
HeLa