Technical Reference #633

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm glass-bottom dishes (MatTek, Ashland, MA)

633.

Lethality To Human Cancer Cells Through Massive Chromosome Loss By Inhibition Of The Mitotic Checkpoint Geert J. P. L. Kops, Daniel R. Foltz, Don W. Cleveland, UCSD, PNAS, 101(23), 8701, (2004)
Link To Paper

Abstract:
A compromised mitotic checkpoint, the primary mechanism for ensuring that each new cell receives one copy of every chromosome, has been implicated as a contributor to carcinogenesis.

Keywords:
carcinogenesis, chromosomally instable colorectal cancer cells, proliferation

Materials & Methods:
HeLa cells seeded on 35-mm glass-bottom dishes (MatTek, Ashland, MA) were transfected with pH2BEYFP and the indicated siRNA plasmids in a ratio of 1:10.

Microscopic Technique
Inverted Microscopy

Cell Type(s)
HeLa