Technical Reference #632

632.

Arp23 Complex-Deficient Mouse Fibroblasts Are Viable And Have Normal Leading-Edge Actin Structure And Function Alessia Di Nardo; Gregor Cicchetti; Herve Falet; John H. Hartwig; Thomas P. Stossel; and David J. Kwiatkowski, Brigham & Women's Hospital, PNAS, 102(632), (2005)
Link To Paper

Abstract:
RNA interference silencing of up to 90% of Arp3 protein expression a major subunit of the Arp2 3 complex proportionately decreases the intracellular motility of Listeria monocytogenes and actin nucleation activity ascribable to the Arp2 3 complex in mou

Keywords:
RNA interference; motility; ruffling

Materials & Methods:
Cells were seeded on 35 mm glass-bottom dishes (MatTek Ashland MA) and microinjected with 1 pg of CFP-CA or CFP-VCA vector DNA by using a MO-150 micromanipulator and an IM200 pressure injector (Narishige Tokyo).

Microscopic Technique
Video Microscopy, Electron microscopy

Cell Type(s)
embryonic fibroblasts