Technical Reference #631

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
collagen-coated 35-mm glass bottom dishes (MatTek, Ashland, MA)

631.

Differential Kinetic And Spatial Patterns Of ?-Arrestin And G Protein-Mediated Erk Activation By The Angiotensin Ii Receptor Seungkirl Ahn, Sudha K. Shenoy, Huijun Wei, and Robert J. Lefkowitz, HHMI, The The Journal of Biological Chemistry, 279(34), 35519, (2004)
Link To Paper

Abstract:
The seven-membrane-spanning angiotensin II type 1A receptor activates the mitogen-activated protein kinases extracellular signal-regulated kinases 1 and 2 (ERK1/2) by distinct pathways dependent on either G protein (likely Gq/G11) or -arrestin2. Here we

Keywords:
angiotensin II type 1A receptor, G protein, B-arrestin2, nuclear translocation, cytoplasmic endosomal vesicles

Materials & Methods:
One day after transfection, cells were divided into collagen-coated 35-mm glass bottom dishes (MatTek, Ashland, MA) for confocal microscopy.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEK-293