Technical Reference #631

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
collagen-coated 35-mm glass bottom dishes (MatTek; Ashland; MA)

631.

Differential Kinetic And Spatial Patterns Of Β-Arrestin And G Protein-Mediated Erk Activation By The Angiotensin Ii Receptor Seungkirl Ahn; Sudha K. Shenoy; Huijun Wei; and Robert J. Lefkowitz, HHMI, The The Journal of Biological Chemistry, 279(631), (2004)
Link To Paper

Abstract:
The seven-membrane-spanning angiotensin II type 1A receptor activates the mitogen-activated protein kinases extracellular signal-regulated kinases 1 and 2 (ERK1/2) by distinct pathways dependent on either G protein (likely Gq/G11) or -arrestin2. Here we

Keywords:
angiotensin II type 1A receptor; G protein; B-arrestin2; nuclear translocation; cytoplasmic endosomal vesicles

Materials & Methods:
One day after transfection cells were divided into collagen-coated 35-mm glass bottom dishes (MatTek Ashland MA) for confocal microscopy.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEK-293