Technical Reference #630

630.

Mutant Protein Kinase C Gamma Found In Spinocerebellar Ataxia Type 14 Is Susceptible To Aggregate And Cause Cell Death Takahiro Seki, Naoko Adachi, Yoshitaka Ono, Hideki Mochizuki, Keiko Hiramoto, Taku Amano, Hiroaki Matsubayashi, Masayasu Matsumoto, Hideshi Kawakami, Naoaki Saito and Norio Sakai, Hiroshima University, The Journal of Biological Chemistry, 280(32), 29097, (2005)
Link To Paper

Abstract:
Spinocerebellar ataxia type 14 (SCA14) is an autosomal dominant neurodegenerative disease characterized by various symptoms including cerebellar ataxia. Recently, several missense mutations in protein kinase C ( PKC) gene have been found in different SCA

Keywords:
Spinocerebellar ataxia type 14 (SCA14), protein kinase C ( PKC) gene, aggregate, cytoplasm, cell death, cytotoxicity

Materials & Methods:
CHO cells (1×105 cells) were spread onto poly-D-lysine-coated glass bottom culture dishes (MatTek Corp., Ashland, MA) and were transfected with 2.5 μg of plasmid by lipofection.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
CHO