Technical Reference #630

630.

Mutant Protein Kinase C Gamma Found In Spinocerebellar Ataxia Type 14 Is Susceptible To Aggregate And Cause Cell Death Takahiro Seki; Naoko Adachi; Yoshitaka Ono; Hideki Mochizuki; Keiko Hiramoto; Taku Amano; Hiroaki Matsubayashi; Masayasu Matsumoto; Hideshi Kawakami; Naoaki Saito and Norio Sakai, Hiroshima University, The Journal of Biological Chemistry, 280(630), (2005)
Link To Paper

Abstract:
Spinocerebellar ataxia type 14 (SCA14) is an autosomal dominant neurodegenerative disease characterized by various symptoms including cerebellar ataxia. Recently several missense mutations in protein kinase C ( PKC) gene have been found in different SCA

Keywords:
Spinocerebellar ataxia type 14 (SCA14); protein kinase C ( PKC) gene; aggregate; cytoplasm; cell death; cytotoxicity

Materials & Methods:
CHO cells (1×105 cells) were spread onto poly-D-lysine-coated glass bottom culture dishes (MatTek Corp. Ashland MA) and were transfected with 2.5 μg of plasmid by lipofection.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
CHO