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Mutant
Protein Kinase C Gamma Found In Spinocerebellar Ataxia Type 14 Is Susceptible To Aggregate And
Cause Cell Death |
Takahiro
Seki, Naoko Adachi, Yoshitaka Ono, Hideki Mochizuki, Keiko Hiramoto, Taku Amano, Hiroaki Matsubayashi,
Masayasu Matsumoto, Hideshi Kawakami, Naoaki Saito and Norio Sakai |
Spinocerebellar
ataxia type 14 (SCA14), protein kinase C?? (??PKC) gene, aggregate, cytoplasm, cell death, cytotoxicity |
CHO cells (1×105 cells) were
spread onto poly-D-lysine-coated glass bottom culture dishes(MatTek Corp., Ashland, MA) and
were transfected with 2.5 µg of plasmid by lipofection.
|
confocal
laser scanning fluorescent microscopy |
chinese
hamster |
|
CHO |
| |
Spinocerebellar ataxia type 14 (SCA14) is an autosomal
dominant neurodegenerative disease characterized by various symptoms including cerebellar ataxia.
Recently, several missense mutations in protein kinase C ( PKC) gene have been found in different
SCA14 families. To elucidate how the mutant PKC causes SCA14, we examined molecular properties
of 7 mutant (H101Y, G118D, S119P, S119F, Q127R, G128D and F643L) PKC fused with |
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