Technical Reference #629

629.

Analysis Of Transient Behavior In Complex Trajectories: Application To Secretory Vesicle Dynamics Sebastien Huet, Erdem Karatekin, Viet Samuel Tran, Isabelle Fanget, Sophie Cribier, Jean-Pierre Henry, Universite´ Paris, Biophysical Journal, 91, 3543, (2006)
Link To Paper

Abstract:
Analysis of trajectories of dynamical biological objects, such as breeding ants or cell organelles, is essential to reveal the interactions they develop with their environments. Many previous works used a global characterization based on parameters calcul

Keywords:
transient behavior, stalled periods, diffusion coefficient, secretory vesicle dynamics, human carcinoid BON cells, dynamical subplasmalemmal organization

Materials & Methods:
All TIRF experiments were performed on a BON NPY-GFP clone, named BC6, which was selected and plated on uncoated glass-bottom dishes (P50G-1.5-14-F, MatTek Cultureware, Ashland, MA).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
BON, BON NPY-GFP clone, named BC6