Technical Reference #629

629.

Analysis Of Transient Behavior In Complex Trajectories: Application To Secretory Vesicle Dynamics Sebastien Huet; Erdem Karatekin; Viet Samuel Tran; Isabelle Fanget; Sophie Cribier; Jean-Pierre Henry, Universite´ Paris, Biophysical Journal, 91(629), (2006)
Link To Paper

Abstract:
Analysis of trajectories of dynamical biological objects such as breeding ants or cell organelles is essential to reveal the interactions they develop with their environments. Many previous works used a global characterization based on parameters calcul

Keywords:
transient behavior; stalled periods; diffusion coefficient; secretory vesicle dynamics; human carcinoid BON cells; dynamical subplasmalemmal organization

Materials & Methods:
All TIRF experiments were performed on a BON NPY-GFP clone named BC6 which was selected and plated on uncoated glass-bottom dishes (P50G-1.5-14-F MatTek Cultureware Ashland MA).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
BON, BON NPY-GFP clone, named BC6