Technical Reference #628

628.

Motor Neurons Are Selectively Vulnerable To Ampa/Kainate Receptor-Mediated Injury In Vitro Sean G. Carriedo; Hong Z. Yin; and John H. Weiss, UC-Irvine, The Journal of Neuroscience, 16(628), (1996)
Link To Paper

Abstract:
The nonphosphorylated neurofilament marker SMI-32 stains motor neurons in spinal cord slices and stains a subset of cultured spinal neurons [“large SMI-32(1) neurons”] which have a morphology consistent with motor neurons identified in vitro: large cell

Keywords:
cell culture; AMPA; kainate; glutamate; motor neuron; calcium; SMI-32; peripherin; ChAT; neurotoxicity; cobalt; calcium imaging

Materials & Methods:
For Ca21 imaging studies cultures were prepared as described above except that cells were plated on poly-L-lysine-coated glass-bottomed plates (Plastek Cultureware Ashland MA).

Microscopic Technique
Fluorescence Microscopy, Inverted Fluorescence Microscopy

Cell Type(s)
Spinal cord cultures