Technical Reference #628

628.

Motor Neurons Are Selectively Vulnerable To Ampa/Kainate Receptor-Mediated Injury In Vitro Sean G. Carriedo, Hong Z. Yin, and John H. Weiss, UC-Irvine, The Journal of Neuroscience, 16(13), 4070, (1996)
Link To Paper

Abstract:
The nonphosphorylated neurofilament marker SMI-32 stains motor neurons in spinal cord slices and stains a subset of cultured spinal neurons [“large SMI-32(1) neurons”], which have a morphology consistent with motor neurons identified in vitro: large cell

Keywords:
cell culture, AMPA, kainate, glutamate, motor neuron, calcium, SMI-32, peripherin, ChAT, neurotoxicity, cobalt, calcium imaging

Materials & Methods:
For Ca21 imaging studies, cultures were prepared as described above except that cells were plated on poly-L-lysine-coated glass-bottomed plates (Plastek Cultureware, Ashland, MA).

Microscopic Technique
Fluorescence Microscopy, Inverted Fluorescence Microscopy

Cell Type(s)
Spinal cord cultures