Technical Reference #627

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottomed dishes (Mattek Cultureware, Ashland, MA)

627.

Rapid Ca21 Entry Through Ca21-Permeable Ampa/Kainate Channels Triggers Marked Intracellular Ca21 Rises And Consequent Oxygen Radical Production Sean G. Carriedo, Hong Zhen Yin, Stefano L. Sensi, John H. Weiss, UC-Irvine, The Journal of Neuroscience, 18(19), 7728, (1998)
Link To Paper

Abstract:
The widespread neuronal injury that results after brief activation of highly Ca21-permeable NMDA channels may, in large part, reflect mitochondrial Ca21 overload and the consequent production of injurious oxygen radicals. In contrast, AMPA/kainate recepto

Keywords:
cell culture, glutamate, AMPA, kainate, NMDA, cobalt, hydroethidine, calcium imaging, fura-2, fura-2FF, free radicals, superoxide, tetramethylrhodamine ethylester

Materials & Methods:
Cultures were plated on glass-bottomed dishes (Mattek Cultureware, Ashland, MA) and mounted to a stage adapter on an inverted microscope (Nikon Diaphot, Tokyo, Japan).

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Neocortical