Technical Reference #627

627.

Rapid Ca21 Entry Through Ca21-Permeable Ampa/Kainate Channels Triggers Marked Intracellular Ca21 Rises And Consequent Oxygen Radical Production Sean G. Carriedo; Hong Zhen Yin; Stefano L. Sensi; John H. Weiss, UC-Irvine, The Journal of Neuroscience, 18(627), (1998)
Link To Paper

Abstract:
The widespread neuronal injury that results after brief activation of highly Ca21-permeable NMDA channels may in large part reflect mitochondrial Ca21 overload and the consequent production of injurious oxygen radicals. In contrast AMPA/kainate recepto

Keywords:
cell culture; glutamate; AMPA; kainate; NMDA; cobalt; hydroethidine; calcium imaging; fura-2; fura-2FF; free radicals; superoxide; tetramethylrhodamine ethylester

Materials & Methods:
Cultures were plated on glass-bottomed dishes (Mattek Cultureware Ashland MA) and mounted to a stage adapter on an inverted microscope (Nikon Diaphot Tokyo Japan).

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Neocortical