Technical Reference #626

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm dishes containing a 1-cm glass-bottomed center well (Plastek Cultureware; Ashland; MA)

626.

Nuclear Localization Of The 82-Kda Form Of Human Choline Acetyltransferase Manuel C. Resendes; Tomas Dobransky; Stephen S. G. Ferguson; R. Jane Rylett, University of Western Ontario, The Journal of Biological Chemistry, 274(626), (1999)
Link To Paper

Abstract:
Choline acetyltransferase is the enzyme catalyzing synthesis of the neurotransmitter acetylcholine in cholinergic neurons.

Keywords:
Choline acetyltransferase; enzyme catalyzing synthesis; brain; spinal cord; amino acid; cell nucleus

Materials & Methods:
For confocal microscopy cells were replated at 24 h after transfection onto 35-mm dishes containing a 1-cm glass-bottomed center well (Plastek Cultureware Ashland MA) in growth medium supplemented with 20 mM HEPES buffer pH 7.4.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEK-293