Technical Reference #625

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
24-well plates or glass-bottomed dishes (Plastek Cultureware, Ashland,MA)

625.

Preferential Zn21 Influx Through Ca21-Permeable Ampaykainate Channels Triggers Prolonged Mitochondrial Superoxide Production Stefano L. Sensi, Hong Z. Yin, Sean G. Carriedo, Shyam S. Rao, John H. Weiss, UC-Irvine, PNAS, 96, 2414, (1999)
Link To Paper

Abstract:
Synaptically released Zn21 can enter and cause injury to postsynaptic neurons.

Keywords:
N-methyl-D-asparte, kainate, hydroethidine oxidation, reactive oxygen species (ROS), mitochondrial electron transport blocker rotenone

Materials & Methods:
Briefly, dissociated mixed neocortical cell suspensions were prepared...on previously established astrocytic monolayers in either 24-well plates or glass-bottomed dishes (Plastek Cultureware, Ashland,MA).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Neocortical cell suspensions