Technical Reference #624

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm experimental chamber with a glass bottom (Plastek Cultureware, Ashland, MA).

624.

Transgenic Cystic Fibrosis Mice Exhibit Reduced Early Clearance Of Pseudomonas Aeruginosa From The Respiratory Tract Torsten H. Schroeder, Nina Reiniger, Gloria Meluleni, Martha Grout, Fadie T. Coleman, Gerald B. Pier, Brigham & Women's Hospital, Harvard Medical School, University of Tuebingen, The Journal of Immunology, 166, 7411, (2001)
Link To Paper

Abstract:
The cystic fibrosis (CF) transmembrane conductance regulator (CFTR) has been proposed to be an epithelial cell receptor for Pseudomonas aeruginosa involved in bacterial internalization and clearance from the lung. We evaluated the role of CFTR in clearing

Keywords:
cystic fibrosis, Pseudomonas aeruginosa, CFTR-mediated bacterial ingestion

Materials & Methods:
The tracheas were then placed in F12 medium and transferred into a 35-mm experimental chamber with a glass bottom (Plastek Cultureware, Ashland, MA).

Microscopic Technique
Confocal Microscopy

Cell Type(s)
P. aeruginosa strain PAO1