Technical Reference #623

623.

Activation Of The Leptin Receptor By A Ligand-Induced Conformational Change Of Constitutive Receptor Dimers Cyril Couturier, Ralf Jockers, Universite Rene´ Descartes, The The Journal of Biological Chemistry, 278(29), 26605, (2003)
Link To Paper

Abstract:
Binding of leptin to the leptin receptor is crucial for body weight and bone mass regulation in mammals. Leptin receptors were shown to exist as dimers, but the role of dimerization in receptor activation remains unknown. Using a quantitative Bioluminesce

Keywords:
leptin, Bioluminescence Resonance Energy Transfer approach, leptin receptor isoform

Materials & Methods:
COS-7 cells transfected with either OBRs–YFP or OB-Rl–YFP expression plasmids were grown on 35-mm glass-bottomed microwell dishes (Plastek Cultureware, MatTek Corp., Ashland, MA).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HEK-293