Technical Reference #622

622.

Anthrax Lethal Toxin Paralyzes Neutrophil Actin-Based Motility Russell L. During; Wei Li; Binghua Hao; Joyce M. Koenig; David S. Stephens; Conrad P. Quinn; Frederick S. Southwick, University of Florida, The Journal of Infectious Diseases, 192(622), (2005)
Link To Paper

Abstract:
Bacillus anthracis causes high-level bacteremia strongly suggesting paralysis of the innate immune system. We have examined the effects of anthrax lethal toxin (LT) on human neutrophil chemotaxis a process that requires actin filament assembly. Polymorp

Keywords:
Bacillus anthracis; paralysis; innate immune system; anthrax lethal toxin (LT); neutrophil chemotaxis; Polymorphonuclear neutrophils (PMNs); apoptosis; necrosis; chemokinesis

Materials & Methods:
Untreated and LT-treated PMNs (1105 cells in 2 mL of RPMI) were added to 35-mm glass-bottom microwell dishes (MatTek Cultureware) coated with 0.1% fibronectin (Sigma).

Microscopic Technique
Inverted Microscopy

Cell Type(s)
PMNs