Technical Reference #622

622.

Anthrax Lethal Toxin Paralyzes Neutrophil Actin-Based Motility Russell L. During, Wei Li, Binghua Hao, Joyce M. Koenig, David S. Stephens, Conrad P. Quinn, Frederick S. Southwick, University of Florida, The Journal of Infectious Diseases, 192, 839, (2005)
Link To Paper

Abstract:
Bacillus anthracis causes high-level bacteremia, strongly suggesting paralysis of the innate immune system. We have examined the effects of anthrax lethal toxin (LT) on human neutrophil chemotaxis, a process that requires actin filament assembly. Polymorp

Keywords:
Bacillus anthracis, paralysis, innate immune system, anthrax lethal toxin (LT), neutrophil chemotaxis, Polymorphonuclear neutrophils (PMNs), apoptosis, necrosis, chemokinesis

Materials & Methods:
Untreated and LT-treated PMNs (1105 cells in 2 mL of RPMI) were added to 35-mm glass-bottom microwell dishes (MatTek Cultureware) coated with 0.1% fibronectin (Sigma).

Microscopic Technique
Inverted Microscopy

Cell Type(s)
PMNs