Technical Reference #621

621.

Localization And Functional Interrelationships Among Cytosolic Group Iv, Secreted Group V, And Ca2+-Independent Group Vi Phospholipase A2S In P388D1 Macrophages Using Gfp/Rfp Constructs Yasuhito Shirai, Jesus Balsinde, Edward A. Dennis, UCSD, Biochimica et Biophysica Acta, 1735, 121, (2005)
Link To Paper

Abstract:
P388D1 cells exposed to bacterial lipopolysaccharide (LPS) mobilize arachidonic acid (AA) for prostaglandin synthesis in two temporally distinct pathways. The ‘‘immediate pathway’’ is triggered within minutes by receptor agonists such as platelet-activati

Keywords:
phospholipase A2, arachidonic acid, macrophage, platelet-activating factor, lipopolysaccharide, prostaglandin

Materials & Methods:
An appropriate number of P388D1 cells transiently or stably expressing the PLA2 fusion proteins were placed on a glass-bottomed dish (MetTek, Ashland, MA) and cultured overnight.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
P388D1