Technical Reference #620

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass bottom dish (Matek Corporation)

620.

A Role For Aqp5 In Activation Of Trpv4 By Hypotonicity: Concerted Involvement Of Aqp5 And Trpv4 In Regulation Of Cell Volume Recovery Xibao Liu; Bidhan Bandyopadhyay; Tetsuji Nakamoto; Brij Singh; Wolfgang Liedtke; James E. Melvin; and Indu Ambudkar, National Institutes of Health, The Journal of Biological Chemistry, 281(620), (2006)
Link To Paper

Abstract:
Regulation of cell volume in response to changes in osmolarity is critical for cell function and survival. However the molecular basis of osmosensation and regulation of cell volume are not clearly understood.

Keywords:
cell volume; osmosensation; regulatory volume decrease (RVD); transient receptor potential vanalloid 4 (TRPV4); aquaporin 5 (AQP5); hypotonicity

Materials & Methods:
Freshly isolated salivary gland cells were loaded with fura 2 for 45-60 min at 30 °C and allowed to attach to glass bottom dish (Matek Corporation).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
SALIVARY