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MATERIALS & METHODS

MICROSCOPIC TECHNIQUES SPECIES MORPHOLOGY CELL LINE
619 The Chemical Species of Aluminum Influences Its Paracellular Flux across and Uptake into Caco-2 Cells, a Model of
Gastrointestinal Absorption
Yuzhao Zhou and Robert A. Yokel aluminum absorption, Caco-2 cells, chemical species, flux, paracellular pathway, uptake

Caco-2 cells were seeded at a density of 1 3 105 cells/cm2 onto 35-mmdiameter
glass-bottomed coverslip dishes (MatTeck Corp.) and cultured for 5 to
7 days.

confocal laser microscopy Caco-2
  Aluminum (Al) can cause neurotoxicity, a low-turnover osteomalacia, and microcytic anemia. To test the null hypothesis that the chemical form (species) of Al does not influence its mechanism or rate of absorption from the gastrointestinal tract, Al flux across and uptake into Caco-2 cells was investigated. Caco-2 cells were grown on porous membranes mounted in vertical diffusion chambers or in 35-mm-diameter plastic cell culture dishes. When 8 mM 27Al  

 

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