Technical Reference #618

618.

Regulation Of Sp2 Dna-Binding Activity And Trans-Activation K. Scott Moorefield, North Carolina State University, Electronic Thesis- NCSU, 65, (2005)
Link To Paper

Abstract:
Regulated transcription requires the collaboration of a variety of transcription factors, including sequence-specific DNA-binding proteins. The Sp-family of DNA-binding proteins governs the expression of a wide variety of mammalian genes, including housek

Keywords:
transcription, Sp-family of DNA-binding proteins, trans-activation

Materials & Methods:
For confocal microscopy, 2-4 x 104 COS-1 cells were plated in 35 mm glass-bottom dishes (MatTek Corp., Ashland, MA) and cultured overnight at 37 °C in DMEM supplemented with heat-inactivated FBS, and 50 µg/ml Pipracil at 37 °C under 5% CO2.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
COS-1