Glass Bottom Dishes - specialized petri dishes for imaging cell cultures using high resolution microscopes

TR	TITLE	AUTHORS	KEYWORDS	MATERIALS & METHODS	MICROSCOPIC TECHNIQUES	SPECIES	MORPHOLOGY	CELL LINE
617	Analysis of Toll-Like Receptor 4 Signal Transduction and IRF3 Activation in the Innate Immune Response: A Dissertation	Daniel Rowe	immune system, pattern recognition receptors (PRRs), Toll-like receptors (TLRs), interferon regulatory factor 3 (IRF3), TLR signal transduction, type 1 interferon, IKB kinase epsilon (IKKE), TANK-binding kinase- l (TBK1), MyD88	IR3-GFP-expressing HEK293 cells were plated on 35 mm glass-bottom sterile tissue culture dishes (Mattek Co. , Ashland, MA) and transiently transfected with l g Flagtagged IKK&, TBK1, IKKjJ, and visualized 48 h later by confocal microscopy.	immunofluorescence and confocal microscopy			HEK-293
	Over the last decade, the innate immune system has been the subject of extensive research. Often overlooked by the robustness and specificity of the adaptive immune system, the innate immune system is proving to be just as complex. The identification of several families of pattern recognition receptors (PRRs) has revealed an ancient yet multifaceted system of proteins that are responsible for initiating host defense. A wide array of pathogens, from virus