Technical Reference #616

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm glass bottom dishes (MatTek, Ashland, MA)

616.

The Legionella Pneumophila Effector Protein Drra Is A Rab1 Guanine Nucleotide-Exchange Factor Takahiro Murata, Anna Delprato, Alyssa Ingmundson, Derek K. Toomre, David G. Lambright and Craig R. Roy, Yale University, Nature Cell Biology, 8(9), 977, (2006)
Link To Paper

Abstract:
The intracellular pathogen Legionella pneumophila avoids fusion with lysosomes and subverts membrane transport from the endoplasmic reticulum to create an organelle that supports bacterial replication1,2. Transport of endoplasmic reticulumderived vesicles

Keywords:
Legionella pneumophila, membrane transport, endoplasmic reticulum, bacterial replication, Golgi apparatus, GTPases, vesicular transport

Materials & Methods:
For multi-channel TIRFM, CHO Fc?RII cells grown in 35-mm glass bottom dishes (MatTek, Ashland, MA)�

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
CHO