Technical Reference #615

615.

Dendritic Cell Maturation Triggers Retrograde Mhc Class Ii Transport From Lysosomes To The Plasma Membrane Amy Chow; Derek Toomre; Wendy Garrett & Ira Mellman, Yale University, Nature, 418(615), (2002)
Link To Paper

Abstract:
Central to the initiation of immune responses is recognition of peptide antigen by T lymphocytes. The cell biology of dendritic cells makes them ideally suited for the essential process of antigen presentation1. Their life cycle includes several stages ch

Keywords:
peptide antigen; T lymphocytes; antigen presentation; major histocompatibility complex class II molecules (MHC II); endocytosis; green fluorescent protein (GFP)

Materials & Methods:
To label lysosomes day 4–5 immature dendritic cells were…plated for 30min on poly-L-lysine pre-coated number 1.5 coverslips attached to 35-mm dishes (MatTek).

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Dendritic