Technical Reference #614

614.

Gbf1, A Cis-Golgi And Vtcs-Localized Arf-Gef, Is Implicated In Er-To-Golgi Protein Traffic Xinhua Zhao, Alejandro Claude, Justin Chun, David J. Shields, John F. Presley and Paul Melançon,, University of Alberta, The Journal of Cell Biology, 119, 3725, (2006)
Link To Paper

Abstract:
The formation and maturation of membrane carriers that transport cargo from the ER to the Golgi complex involves the sequential action of the coat protein complexes COPII and COPI. Recruitment of COPI to nascent carriers requires activation of ADP-ribosyl

Keywords:
GBF1, ERGIC, VTCs, ARF-GEF, Brefeldin A, Protein traffic

Materials & Methods:
For Fig. 7, NRK-GFP-GBF1 cells were grown on Plastek Cultureware glass bottom microwell dishes (MatTek Corp) and imaged on the temperature-controlled stage of a Zeiss Axiovert 200M microscope equipped with an UltraVIEW ERS 3E spinning disk.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
NRk