Technical Reference #612

612.

Chromosome Nondisjunction Yields Tetraploid Rather Than Aneuploid Cells In Human Cell Lines Qinghua Shi & Randall W. King, Harvard Medical School, Nature, 437, 1041, (2005)
Link To Paper

Abstract:
Although mutations in cell cycle regulators or spindle proteins can perturb chromosome segregation1–7, the causes and consequences of spontaneous mitotic chromosome nondisjunction in human cells are not well understood.

Keywords:
mutation, spontaneous mitotic chromosome nondisjunction, mitosis, aneuploid, tetraploid, chromosome mis-segregation, furrow regression, cancer

Materials & Methods:
HeLa or N/TERT-1 cells expressing GFP–histone 2B were grown on gridded coverglass bottom dishes (MatTek, CatNo. P35G-1.5-7-C-grid) for 26 h (HeLa, 2 £ 105 cells per dish) or 3 d (N/TERT-1, 4 £ 104 cells per dish) before imaging.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HeLa