Technical Reference #611

611.

Ca2+ Shuttling Between Endoplasmic Reticulum And Mitochondria Underlying Ca2Þ Oscillations Kiyoaki Ishii; Kenzo Hirose & Masamitsu Iino, University of Tokyo, European Molecular Biology Organization Reports, 7(611), (2006)
Link To Paper

Abstract:
Although many cell functions are regulated by Ca2þ oscillations induced by a cyclic release of Ca2þ from intracellular Ca2þ stores the pacemaker mechanism of Ca2þ oscillations remains to be explained.

Keywords:
calcium oscillation; endoplasmic reticulum; GFP; imaging; mitochondria

Materials & Methods:
HeLa cells transduced with retroviruses encoding the indicators and cultured in glass-bottomed dish (MatTek) were loaded at room temperature (23 1C–25 1C) with 5 mM indo-5F AM or fura-2 AM in physiological salt solution…

Microscopic Technique
Fluorescence Microscopy, Inverted Fluorescence Microscopy

Cell Type(s)
HeLa