Technical Reference #609

609.

Dynamic Regulation Of Tec Kinase Localization In Membraneproximal Vesicles Of A T Cell Clone Revealed By Total Internal Reflection Fluorescence And Confocal Microscopy Lawrence P Kane, Simon C. Watkins, University of Pittsburgh, The The Journal of Biological Chemistry, 280(23), 21950, (2005)
Link To Paper

Abstract:
Tec family tyrosine kinases are key regulators of lymphocyte activation and effector function. Several Tec family kinases (Tec, Itk, Rlk/Txk) are expressed in T cells, but it is still not clear to what degree these are redundant or have unique functions.

Keywords:
tyrosine kinases, lymphocyte activation, effector function, total internal reflection fluorescence microscopy, early endosomal antigen 1 marker

Materials & Methods:
Glass bottom 35-mm cell culture dishes (MatTek, Ashland, MA) were precoated overnight at 4 °C with antibodies to CD3 and CD28 or LFA-1 and diluted to 10 g/ml in phosphate-buffered saline.

Microscopic Technique
Fluorescence Microscopy, Total Internal Reflection Fluorescence Microscopy

Cell Type(s)
D10