Technical Reference #606

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35 mm tissue culture dishes (MatTek Corp.; Ashland; MA)

606.

Prospective Cell Sorting Of Embryonic Rat Neural Stem Cells And Neuronal And Glial Progenitors Reveals Selective Effects Of Basic Fibroblast Growth Factor And Epidermal Growth Factor On Self-Renewal And Differentiation Dragan Maric; Irina Maric; Yoong Hee Chang; and Jeffery L. Barker, National Institutes of Health, The Journal of Neuroscience, 23(606), (2003)
Link To Paper

Abstract:
We directly isolated neural stem cells and lineage-restricted neuronal and glial progenitors from the embryonic rat telencephalon using a novel strategy of surface labeling and fluorescence-activated cell sorting.

Keywords:
fluorescence-activated cell sorting; negative selection; positive selection; neural stem cells; lineage-restricted progenitors; growth factors; calcium imaging

Materials & Methods:
…coverslips which were photo-etched with an alphanumeric grid (Bellco Glass Inc. Vineland NJ) and preglued to 35 mm tissue culture dishes (MatTek Corp. Ashland MA).

Microscopic Technique
Fluorescence Microscopy, Inverted Fluorescence Microscopy

Cell Type(s)
E13