Technical Reference #604

604.

Ionized Intracellular Calcium Concentration Predicts Excitotoxic Neuronal Death: Observations With Low-Affinity Fluorescent Calcium Indicators Krzysztof Hyrc, Shawn D. Handran, Steven M. Rothman, and Mark P. Goldberg, Washington University, The The Journal of Neuroscience, 17, 6670, (1997)
Link To Paper

Abstract:
Cytosolic calcium ([Ca21]i ) is an important mediator of neuronal signal transduction, participating in diverse biochemical reactions that elicit changes in synaptic efficacy, metabolic rate, and gene transcription. Excessive [Ca21]i also has been implica

Keywords:
AMPA, calcium, excitotoxicity, fura-2, glutamate, kainate, NMDA, videomicroscopy

Materials & Methods:
Cells were plated (0.5 hemisphere per dish) onto glass-bottom 35 mm dishes (Mattek, Ashland, MA) previously coated with a mixture of poly-D-lysine (0.5 mg/ml) and laminin (4 mg/ml).

Microscopic Technique
Video Microscopy

Cell Type(s)
Neocortical