Technical Reference #604
Glass Bottom Culture Dishes
This study used MatTek product(s):
Citation in paper containing MatTek reference:
glass-bottom 35 mm dishes (Mattek; Ashland; MA) 
604. |
Ionized Intracellular Calcium Concentration Predicts Excitotoxic Neuronal Death: Observations With Low-Affinity Fluorescent Calcium Indicators
Krzysztof Hyrc; Shawn D. Handran; Steven M. Rothman; and Mark P. Goldberg,
Washington University,
The The Journal of Neuroscience,
17(604),
(1997)
Link To Paper
Abstract:
Cytosolic calcium ([Ca21]i ) is an important mediator of neuronal signal transduction participating in diverse biochemical reactions that elicit changes in synaptic efficacy metabolic rate and gene transcription. Excessive [Ca21]i also has been implica Keywords:
AMPA; calcium; excitotoxicity; fura-2; glutamate; kainate; NMDA; videomicroscopy Materials & Methods:
Cells were plated (0.5 hemisphere per dish) onto glass-bottom 35 mm dishes (Mattek Ashland MA) previously coated with a mixture of poly-D-lysine (0.5 mg/ml) and laminin (4 mg/ml). Microscopic Technique
Video Microscopy Cell Type(s)
Neocortical |