Technical Reference #603

603.

A Role For Nr-Cam In The Patterning Of Binocular Visual Pathways Scott E. Williams; Martin Grumet; David R. Colman; Mark Henkemeyer; Carol A. Mason; and Takeshi Sakurai, Columbia University, Neuron, 50(603), (2006)
Link To Paper

Abstract:
Retinal ganglion cell (RGC) axons diverge within the optic chiasm to project to opposite sides of the brain. In mouse contralateral RGCs are distributed throughout the retina whereas ipsilateral RGCs are restricted to the ventrotemporal crescent (VTC).

Keywords:
Retinal ganglion cell (RGC); optic chiasm; cell adhesion; binocular visual pathways

Materials & Methods:
For retina-chiasm cocultures retinal explants were plated onto 12 mm glass coverslip-bottom dishes (MatTek) coated sequentially with 0.01% polyornithine (Sigma) and 10 mg/ml (E14.5) or 20 mg/ml (E17.5) mouse laminin (Invitrogen) for 2 hr each at 37ºC.

Microscopic Technique
Dissecting Microscopy

Cell Type(s)
E14.5