Technical Reference #602

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm glass bottom dishes (MatTek Co., Ashland, MA), coated with poly-D-lysine

602.

Beta-Amyloid-Induced Neurodegeneration And Protection By Structurally Diverse Microtubule-Stabilizing Agents M. L. Michaelis, S. Ansar, Y. Chen, E. R. Reiff, K. I. Seyb, R. H. Himes, K. L. Audus, and G. I. Georg, University of Kansas, The Journal of Pharmacology and Experimental Therapeutics, 312(2), 660, (2005)
Link To Paper

Abstract:
Deposition of -amyloid peptide (A ) and hyperphosphorylation of the protein are associated with neuronal dysfunction and cell death in Alzheimer�s disease. Although the relationship between these two processes is not yet understood, studies have shown

Keywords:
-amyloid peptide (A), hyperphosphorylation, neuronal dysfunction, cell death, Alzheimer's disease, neuronal dystrophy

Materials & Methods:
After the final precipitation step, neurons were suspended in fresh Dulbecco�s modified Eagle�s medium/F-12 (Sigma-Aldrich, St. Louis, MO) with 10% fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA) and plated at a density of 2.5 x 10^5 cells in

Microscopic Technique
Fluorescence Microscopy, Inverted Fluorescence Microscopy

Cell Type(s)
Cortical