Technical Reference #601

601.

Hiv-1 Nef Mediates Post-Translational Down-Regulation And Redistribution Of The Mannose Receptor David J. Vigerust, Brian S. Egan, and Virginia L. Shepherd, Vanderbilt University, Journal of Leukocyte Biology, 77, 525, (2005)
Link To Paper

Abstract:
Human immunodeficiency virus (HIV) has derived a variety of means to evade the host immune response.

Keywords:
macrophages, trafficking, HIV-derived proteins

Materials & Methods:
Transfection of cells for confocal experiments was performed in 35 mm glass-bottom dishes (MatTek, Ashland, MA). Briefly, 5 105 cells were seeded into each dish and incubated overnight.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HeLa