Technical Reference #599

599.

Fluorogenic Phospholipids As Head Group-Selective Reporters Of Phospholipase A Activity Tyler M. Rose and Glenn D. Prestwich, University of Utah, ACS Chemical Biology, 1(599), (2006)
Link To Paper

Abstract:
PLA (phospholipases A) are important mediators of cell signaling generating bioactive fatty acids and LPLs (lysophospholipids). PLA products having different head groups can initiate vastly different types of signaling. Fluorogenic analogues of the PLs (

Keywords:
phospholipases A; cell signaling; lisophospholipids; PLA2 enzymes

Materials & Methods:
Dissociated neurons from 8–16 week-old SCG were plated at a density of one-half ganglion per one 35 mm poly-l-lysine-coated glass bottom petri dish (no. 1.5 MatTek Corp.) and incubated at 37 °C in a 5% CO2 environment in MEM.

Microscopic Technique
Confocal Microscopy, Video rate

Cell Type(s)
SCG neurons