Technical Reference #598

598.

Interaction Of Polycationic Polymers With Supported Lipid Bilayers And Cells: Nanoscale Hole Formation And Enhanced Membrane Permeability Seungpyo Hong, Pascale R. Leroueil, Elizabeth K. Janus, Jennifer L. Peters, Mary-Margaret Kober, Mohammad T. Islam, Bradford G. Orr, James R. Baker, Jr., and Mark M. Banaszak Holl, University of Michigan, Bioconjugate Chemistry, 17(3), 729, (2006)
Link To Paper

Abstract:
Interactions of polycationic polymers with supported 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) lipid bilayers and live cell membranes (KB and Rat2) have been investigated using atomic force microscopy (AFM), cytosolic enzyme assays, confocal lase

Keywords:
polycationic polymers, lipid bilayers, lactate dehydrogenase, luciferase, membrane permeabilit

Materials & Methods:
A concentration of 2 104 cells/mL of Rat2 cells was seeded on MatTek glass bottom Petri dishes (35 mm) and incubated at 37 °C under 5% CO2 for 24 h.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Rat2