Technical Reference #598

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
MatTek glass bottom Petri dishes (35 mm)

598.

Interaction Of Polycationic Polymers With Supported Lipid Bilayers And Cells: Nanoscale Hole Formation And Enhanced Membrane Permeability Seungpyo Hong; Pascale R. Leroueil; Elizabeth K. Janus; Jennifer L. Peters; Mary-Margaret Kober; Mohammad T. Islam; Bradford G. Orr; James R. Baker; Jr.; and Mark M. Banaszak Holl, University of Michigan, Bioconjugate Chemistry, 17(598), (2006)
Link To Paper

Abstract:
Interactions of polycationic polymers with supported 12-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) lipid bilayers and live cell membranes (KB and Rat2) have been investigated using atomic force microscopy (AFM) cytosolic enzyme assays confocal lase

Keywords:
polycationic polymers; lipid bilayers; lactate dehydrogenase; luciferase; membrane permeabilit

Materials & Methods:
A concentration of 2 104 cells/mL of Rat2 cells was seeded on MatTek glass bottom Petri dishes (35 mm) and incubated at 37 °C under 5% CO2 for 24 h.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Rat2