Technical Reference #597

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottom dishes (MatTek, Ashland, MA)

597.

Spatiotemporal Interactions Of Myristoylated Alanine-Rich C Kinase Substrate (Marcks) Protein With The Actin Cytoskeleton And Exocytosis Of Oxytocin Upon Prostaglandin F2A Stimulation Of Bovine Luteal Cells U. Salli, N. Saito, and F. Stormshak, Oregon State University, Biology of Reproduction, 69(6), 2054, (2003)
Link To Paper

Abstract:
In the bovine corpus luteum (CL) phosphorylation of myristoylated alanine-rich C kinase substrate (MARCKS) protein in response to prostaglandin F2a (PGF2a) is correlated with the secretion of oxytocin.

Keywords:
corpus luteum, mechanisms of hormone action, oxytocin, signal transduction

Materials & Methods:
Dispersed luteal cells (106 cells/dish) from each of four heifers were cultured in glass-bottom dishes (MatTek, Ashland, MA) for 24 h before transfection.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Luteal