Technical Reference #597

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottom dishes (MatTek; Ashland; MA)

597.

Spatiotemporal Interactions Of Myristoylated Alanine-Rich C Kinase Substrate (Marcks) Protein With The Actin Cytoskeleton And Exocytosis Of Oxytocin Upon Prostaglandin F2A Stimulation Of Bovine Luteal Cells U. Salli; N. Saito; and F. Stormshak, Oregon State University, Biology of Reproduction, 69(597), (2003)
Link To Paper

Abstract:
In the bovine corpus luteum (CL) phosphorylation of myristoylated alanine-rich C kinase substrate (MARCKS) protein in response to prostaglandin F2a (PGF2a) is correlated with the secretion of oxytocin.

Keywords:
corpus luteum; mechanisms of hormone action; oxytocin; signal transduction

Materials & Methods:
Dispersed luteal cells (106 cells/dish) from each of four heifers were cultured in glass-bottom dishes (MatTek Ashland MA) for 24 h before transfection.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Luteal