Technical Reference #596

596.

Sonic Hedgehog, Secreted By Amacrine Cells, Acts As A Short-Range Signal To Direct Differentiation And Lamination In The Zebrafish Retina Alena Shkumatava, Sabine Fischer, Ferenc Müller, Uwe Strahle and Carl J. Neumann, European Molecular Biology Laboratory, Development, 131(16), 3850, (2004)
Link To Paper

Abstract:
Neurogenesis in the zebrafish retina occurs in several waves of differentiation. The first neurogenic wave generates ganglion cells and depends on hedgehog (hh) signaling activity. Using transgenic zebrafish embryos that express GFP under the control of t

Keywords:
Sonic hedgehog, Atonal homolog 5, Retina, Differentiation, Neurogenesis, Zebrafish

Materials & Methods:
Embryos were then imbedded in a coverslip-bottomed petri dish (MatTek Corporation), in 0.5% low melting point agarose dissolved in embryo medium with 0.02% tricaine.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Embryos